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type iib fibers  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank type iib fibers
    Type Iib Fibers, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type iib fibers/product/Developmental Studies Hybridoma Bank
    Average 99 stars, based on 843 article reviews
    type iib fibers - by Bioz Stars, 2026-03
    99/100 stars

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    (A) Gene targeting strategy for generating Chodl MKO mice. (B, C) Western blot ( B ) and qPCR ( C ) analysis of TA muscle samples from young adult WT or Chodl MKO mice. n=5 mice each group. (D) Growth curve of WT and Chodl MKO mice. n=5 mice each group. (E) Representative images of TA muscles and soleus muscles in young adult WT and Chodl MKO mice. (F) Skeletal muscle tissue weight. TA, Tibialis anterior muscle. SOL, Soleus muscle. n=5 mice each group. (G) H&E staining of young adult WT and Chodl MKO mice TA muscle cross-sections. Scale bar: 50 μm. (H) Average myofiber CSA of TA muscle section. n=3 mice each group. (I) Representative fiber-typing images of soleus muscles. Scale bar: 200 μm. (J) Abundancy of Type I, <t>Type</t> <t>IIa</t> and Type <t>IIb</t> myofibers in soleus muscles of WT and Chodl MKO mice. n=5 mice each group. Data are represented as mean ± SEM; Unpaired Student’s t -test; *** P < 0.001.
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    (A) Gene targeting strategy for generating Chodl MKO mice. (B, C) Western blot ( B ) and qPCR ( C ) analysis of TA muscle samples from young adult WT or Chodl MKO mice. n=5 mice each group. (D) Growth curve of WT and Chodl MKO mice. n=5 mice each group. (E) Representative images of TA muscles and soleus muscles in young adult WT and Chodl MKO mice. (F) Skeletal muscle tissue weight. TA, Tibialis anterior muscle. SOL, Soleus muscle. n=5 mice each group. (G) H&E staining of young adult WT and Chodl MKO mice TA muscle cross-sections. Scale bar: 50 μm. (H) Average myofiber CSA of TA muscle section. n=3 mice each group. (I) Representative fiber-typing images of soleus muscles. Scale bar: 200 μm. (J) Abundancy of Type I, <t>Type</t> <t>IIa</t> and Type <t>IIb</t> myofibers in soleus muscles of WT and Chodl MKO mice. n=5 mice each group. Data are represented as mean ± SEM; Unpaired Student’s t -test; *** P < 0.001.
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    (A) Gene targeting strategy for generating Chodl MKO mice. (B, C) Western blot ( B ) and qPCR ( C ) analysis of TA muscle samples from young adult WT or Chodl MKO mice. n=5 mice each group. (D) Growth curve of WT and Chodl MKO mice. n=5 mice each group. (E) Representative images of TA muscles and soleus muscles in young adult WT and Chodl MKO mice. (F) Skeletal muscle tissue weight. TA, Tibialis anterior muscle. SOL, Soleus muscle. n=5 mice each group. (G) H&E staining of young adult WT and Chodl MKO mice TA muscle cross-sections. Scale bar: 50 μm. (H) Average myofiber CSA of TA muscle section. n=3 mice each group. (I) Representative fiber-typing images of soleus muscles. Scale bar: 200 μm. (J) Abundancy of Type I, <t>Type</t> <t>IIa</t> and Type <t>IIb</t> myofibers in soleus muscles of WT and Chodl MKO mice. n=5 mice each group. Data are represented as mean ± SEM; Unpaired Student’s t -test; *** P < 0.001.
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    (A) Gene targeting strategy for generating Chodl MKO mice. (B, C) Western blot ( B ) and qPCR ( C ) analysis of TA muscle samples from young adult WT or Chodl MKO mice. n=5 mice each group. (D) Growth curve of WT and Chodl MKO mice. n=5 mice each group. (E) Representative images of TA muscles and soleus muscles in young adult WT and Chodl MKO mice. (F) Skeletal muscle tissue weight. TA, Tibialis anterior muscle. SOL, Soleus muscle. n=5 mice each group. (G) H&E staining of young adult WT and Chodl MKO mice TA muscle cross-sections. Scale bar: 50 μm. (H) Average myofiber CSA of TA muscle section. n=3 mice each group. (I) Representative fiber-typing images of soleus muscles. Scale bar: 200 μm. (J) Abundancy of Type I, <t>Type</t> <t>IIa</t> and Type <t>IIb</t> myofibers in soleus muscles of WT and Chodl MKO mice. n=5 mice each group. Data are represented as mean ± SEM; Unpaired Student’s t -test; *** P < 0.001.
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    (A) Gene targeting strategy for generating Chodl MKO mice. (B, C) Western blot ( B ) and qPCR ( C ) analysis of TA muscle samples from young adult WT or Chodl MKO mice. n=5 mice each group. (D) Growth curve of WT and Chodl MKO mice. n=5 mice each group. (E) Representative images of TA muscles and soleus muscles in young adult WT and Chodl MKO mice. (F) Skeletal muscle tissue weight. TA, Tibialis anterior muscle. SOL, Soleus muscle. n=5 mice each group. (G) H&E staining of young adult WT and Chodl MKO mice TA muscle cross-sections. Scale bar: 50 μm. (H) Average myofiber CSA of TA muscle section. n=3 mice each group. (I) Representative fiber-typing images of soleus muscles. Scale bar: 200 μm. (J) Abundancy of Type I, Type IIa and Type IIb myofibers in soleus muscles of WT and Chodl MKO mice. n=5 mice each group. Data are represented as mean ± SEM; Unpaired Student’s t -test; *** P < 0.001.

    Journal: bioRxiv

    Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

    doi: 10.1101/2025.09.09.675211

    Figure Lengend Snippet: (A) Gene targeting strategy for generating Chodl MKO mice. (B, C) Western blot ( B ) and qPCR ( C ) analysis of TA muscle samples from young adult WT or Chodl MKO mice. n=5 mice each group. (D) Growth curve of WT and Chodl MKO mice. n=5 mice each group. (E) Representative images of TA muscles and soleus muscles in young adult WT and Chodl MKO mice. (F) Skeletal muscle tissue weight. TA, Tibialis anterior muscle. SOL, Soleus muscle. n=5 mice each group. (G) H&E staining of young adult WT and Chodl MKO mice TA muscle cross-sections. Scale bar: 50 μm. (H) Average myofiber CSA of TA muscle section. n=3 mice each group. (I) Representative fiber-typing images of soleus muscles. Scale bar: 200 μm. (J) Abundancy of Type I, Type IIa and Type IIb myofibers in soleus muscles of WT and Chodl MKO mice. n=5 mice each group. Data are represented as mean ± SEM; Unpaired Student’s t -test; *** P < 0.001.

    Article Snippet: Primary and secondary antibodies used in this study were follows: Fiber Type I (MYH7, cat# BA-D8, DSHB, USA, 1:100), Fiber Type IIA (MYH2, SC-71, DSHB, USA, 1:100), Fiber Type IIB (Myh4, cat# BF-F3, DSHB, USA, 1:100), PAX7 (DSHB, USA, 1:10), eMyHC (MYH3, DSHB, USA, 1:100), MyoG (DSHB, USA, 1:1000), α-laminin (cat# L9393, MilliporeSigma, USA, 1:1000), MF20 (MYH1E, DSHB, USA, 1:100), Goat anti-Mouse IgG1, Alexa Fluor 568 (cat# A-21124, Thermo Fisher, USA, 1:1000), Goat anti-Mouse IgG2b, Alexa Fluor 647 (cat# A-211242, Thermo Fisher, USA, 1:1000), Goat anti-Mouse IgM, Alexa Fluor 488 (cat# A-21042, Thermo Fisher, USA, 1:1000).

    Techniques: Western Blot, Muscles, Staining